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Data sheet of EGFR (phospho Y1197) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce,IHC-P |
More info about EGFR (phospho Y1197) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10009 |
| Product name: | EGFR (phospho Y1197) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of EGFR. |
| Gene id: | 1956 |
| Gene name: | EGFR |
| Gene alias: | ERBB|ERBB1|HER1|PIG61|mENA |
| Gene description: | epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Y1197 of human EGFR. |
| Protein accession: | P00533;NP_005219 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:4000-1:20000) Western Blot (1:250-1:1500) Immunohistochemistry (5 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | Reactivity occurs against human EGFR pY1197 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human EGFR is minimal by ELISA. |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using EGFR (phospho Y1197) polyclonal antibody (Cat # PAB10009) shows detection of a band at ~170 KDa corresponding to human EGFR (arrowhead). Staining is not seen in unstimulated A-431 cells (Lane 1), but is seen when A-431 cells are stimulated with EGF (50 ng/mL for 15 min) (lane 2). Approximately 30 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1 : 250. Reaction occurred overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. |
| Applications: | ELISA,WB-Ce,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | Occurrence of epidermal growth factor receptors in benign and malignant ovarian tumors and normal ovarian tissues: an immunohistochemical study.Henzen-Logmans SC, van der Burg ME, Foekens JA, Berns PM, Brussee R, Fieret JH, Klijn JG, Chadha S, Rodenburg CJ. J Cancer Res Clin Oncol. 1992;118(4):303-7. |