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Data sheet of EIF3F polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chicken,Chimpanzee,Fruit fly,Human,Mouse,Pufferfishes,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about EIF3F polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10008 |
| Product name: | EIF3F polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of EIF3F. |
| Gene id: | 8665 |
| Gene name: | EIF3F |
| Gene alias: | EIF3S5|eIF3-p47 |
| Gene description: | eukaryotic translation initiation factor 3, subunit F |
| Immunogen: | A synthetic peptide corresponding to amino acids 114-125 of human EIF3F. |
| Protein accession: | O00303 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:10000-1:40000) Western Blot (1:200-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Bovine,Chicken,Chimpanzee,Fruit fly,Human,Mouse,Pufferfishes,Rat |
| Application image: |  |
| Application image note: | Western blot using EIF3F polyclonal antibody (Cat # PAB10008) shows detection of endogenous EIF3F in lysates from both control HeLa cells (lane 1) and HeLa cells transformed with the kinase cdk11 (lane 2). Cdk11 is responsible for phosphorylating EIF3F in vivo. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1 : 200. This antibody recognizes both phosphorylated and non-phosphorylated eIF3f. Personal Communication, Jiaqi Shi, Univ. Arizona, Tucson, AZ. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Structure of cDNAs encoding human eukaryotic initiation factor 3 subunits. Possible roles in RNA binding and macromolecular assembly.Asano K, Vornlocher HP, Richter-Cook NJ, Merrick WC, Hinnebusch AG, Hershey JW. J Biol Chem. 1997 Oct 24;272(43):27042-52. |