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Data sheet of ECT2 (phospho T790) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chicken,Chimpanzee,Dog,Human,Mouse,Rat,Zebra fish |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about ECT2 (phospho T790) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10006 |
| Product name: | ECT2 (phospho T790) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of ECT2. |
| Gene id: | 1894 |
| Gene name: | ECT2 |
| Gene alias: | FLJ10461|MGC138291 |
| Gene description: | epithelial cell transforming sequence 2 oncogene |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding T790 of human ECT2. |
| Protein accession: | NP_060568;Q9H8V3 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:10000-1:40000) Western Blot (1:1000-1:4000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | Reactivity occurs against human ECT2 protein at the pT790 residue. By ELISA This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human ECT2 is minimal by ELISA, but western blot shows specific staining of interphase cell lysates, which are predicted to contain non-phosphorylated ECT2. |
| Reactivity: | Chicken,Chimpanzee,Dog,Human,Mouse,Rat,Zebra fish |
| Application image: |  |
| Application image note: | Western blot using ECT2 (phospho T790) polyclonal antibody (Cat # PAB10006) showsdetection of endogenous phospho-ECT2 (arrowhead) present in cell lysates from interphase (Lane 1) and mitotic (Lane 2) HeLa cells. Despite specific staining of interphase cells, this reagent is believed to be phospho specific based on ELISA resultsusing both phosphorylated and non-phosphorylated immunizing peptide. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1 : 1,000. Personal Communication, Toru Miki,CCR-NCI, Bethesda, MD. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | The tandem BRCT domains of Ect2 are required for both negative and positive regulation of Ect2 in cytokinesis.Kim JE, Billadeau DD, Chen J. J Biol Chem. 2005 Feb 18;280(7):5733-9. Epub 2004 Nov 15. |