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Data sheet of E2F1 (phospho S364) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chimpanzee,Human |
| Host species | Rabbit |
| Applications | ELISA,IHC-P,WB-Tr |
More info about E2F1 (phospho S364) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10004 |
| Product name: | E2F1 (phospho S364) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of E2F1. |
| Gene id: | 1869 |
| Gene name: | E2F1 |
| Gene alias: | E2F-1|RBAP1|RBBP3|RBP3 |
| Gene description: | E2F transcription factor 1 |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S364 of human E2F1. |
| Protein accession: | Q01094;NP_005216 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:20000-1:100000) Western Blot 1:250-1:2000 Immunohistochemistry (2-20 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | Reactivity occurs against human E2F-1 pS364 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human E2F-1 is minimal by ELISA. This antibody does not cross-react with E2F-1 phosphorylated at other sites. |
| Reactivity: | Chimpanzee,Human |
| Application image: |  |
| Application image note: | Immunohistochemistry of E2F1 (phospho S364) polyclonal antibody (Cat # PAB10004) was used at a 10 ug/mL to detect nuclear and occasion-ally cytoplasmic signal in a variety of tissues in-cluding multi-human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear staining between individual tumors, with some tumors showing strong staining. This image shows E2F1 pS364 staining of human breast carcinoma. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA. |
| Applications: | ELISA,IHC-P,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | The central acidic domain of MDM2 is critical in inhibition of retinoblastoma-mediated suppression of E2F and cell growth.Sdek P, Ying H, Zheng H, Margulis A, Tang X, Tian K, Xiao ZX. J Biol Chem. 2004 Dec 17;279(51):53317-22. Epub 2004 Oct 13. |