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Data sheet of Histone H3 (K9/14ac) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | ELISA,ChIP,Dot-Pep,IF |
More info about Histone H3 (K9/14ac) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB0652 |
| Product name: | Histone H3 (K9/14ac) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of histone H3 (acetylated lysines 9 and 14). |
| Immunogen: | A synthetic peptide (conjugated with KLH) containing the acetylated lysines 9 and 14 (or [K9/14ac]) of human histone H3. |
| Form: | Liquid |
| Recommend dilutions: | The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.1% BSA, 0.01% thimerosal) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains thimerosal: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Immunofluorescence with the Histone H3 (K9/14ac) polyclonal antibody (Cat # PAB0652). HeLa cells were stained with antibody directed against modified histone and with DAPI. Cells were methanol fixed for 10 minutes and then blocked with 1% BSA containing PBS. The fixation step stabilises the morphology of the cells and permeabilizes membranes as well. (A) Cells were immunofluorescently labelled with the Histone H3 (K9/14ac) polyclonal antibody (Cat # PAB0652) (diluted 1 : 200 and incubated for 1 hour at room temperature) followed by goat anti-rabbit antibody conjugated to FITC. (B) Nuclei were DAPI stained to label specifically the DNA. In both cases, the nuclei are clearly stained. |
| Applications: | ELISA,ChIP,Dot-Pep,IF |
| Shipping condition: | Dry Ice |
| Publications: | Epigenetic regulation (DNA methylation, histone modifications) of the 11p15 mucin genes (MUC2, MUC5AC, MUC5B, MUC6) in epithelial cancer cells.Vincent A, Perrais M, Desseyn JL, Aubert JP, Pigny P, Van Seuningen I. Oncogene. 2007 Oct 4;26(45):6566-76. Epub 2007 Apr 30. |