Histone H3 (K9ac) polyclonal antibody View larger

Histone H3 (K9ac) polyclonal antibody

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Data sheet of Histone H3 (K9ac) polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityHuman
Host speciesRabbit
ApplicationsELISA,ChIP,Dot-Pep,IF

More info about Histone H3 (K9ac) polyclonal antibody

Brand: Abnova
Reference: PAB0651
Product name: Histone H3 (K9ac) polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of histone H3 (acetylated lysine 9).
Immunogen: A synthetic peptide (conjugated with KLH) containing the acetylated lysine 9 (or [K9ac]) of human histone H3.
Form: Liquid
Recommend dilutions: The optimal working dilution should be determined by the end user.
Storage buffer: In PBS (0.1% BSA, 0.01% thimerosal)
Storage instruction: Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Note: This product contains thimerosal: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Reactivity: Human
Application image: PAB0651-49-1-1.jpg
Application image note: Immunofluorescence with the Histone H3 (K9ac) polyclonal antibody (Cat # PAB0651) .
HeLa cells were stained with antibody directed against modified histone and with DAPI.
Cells were methanol fixed for 10 minutes and then blocked with 1% BSA containing PBS.
The fixation step stabilises the morphology of the cells and permeabilizes membranes as well.
(A) Cells were immunofluorescently labelled with the Histone H3 (K9ac) polyclonal antibody (Cat # PAB0651) (diluted 1 : 200 and incubated for 1 hour at room temperature) followed by goat anti-rabbit antibody conjugated to FITC.
(B) Nuclei were DAPI stained to label specifically the DNA.
In both cases, the nuclei are clearly stained.
Applications: ELISA,ChIP,Dot-Pep,IF
Shipping condition: Dry Ice

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