Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Human |
Host species | Rabbit |
Applications | ELISA,ChIP,ChIP/Chip,Dot-Pep,IF |
Brand: | Abnova |
Reference: | PAB0650 |
Product name: | Histone H3 (K4me3) polyclonal antibody |
Product description: | Rabbit polyclonal antibody raised against synthetic peptide of histone H3 (trimethylated lysine 4). |
Immunogen: | A synthetic peptide (conjugated with KLH) containing the trimethylated lysine 4 (or [K4me3]) of human histone H3. |
Form: | Liquid |
Recommend dilutions: | The optimal working dilution should be determined by the end user. |
Storage buffer: | In PBS (0.1% BSA, 0.01% thimerosal) |
Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
Note: | This product contains thimerosal: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Product type: | Primary antibodies |
Host species: | Rabbit |
Antigen species / target species: | Human |
Reactivity: | Human |
Application image: | ![]() |
Application image note: | Immunofluorescence with the Histone H3 (K4me3) polyclonal antibody (Cat # PAB0650). HeLa cells were stained with antibody directed against modified histone and with DAPI. Cells were methanol fixed for 10 minutes and then blocked with 1% BSA containing PBS. The fixation step stabilises the morphology of the cells and permeabilizes membranes as well. (A) Cells were immunofluorescently labelled with the Histone H3 (K4me3) polyclonal antibody (Cat # PAB0650) (diluted 1 : 200 and incubated for 1 hour at room temperature) followed by goat anti-rabbit antibody conjugated to FITC. (B) Nuclei were DAPI stained to label specifically the DNA. In both cases, the nuclei are clearly stained. |
Applications: | ELISA,ChIP,ChIP/Chip,Dot-Pep,IF |
Shipping condition: | Dry Ice |