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SRC (phospho Y215) polyclonal antibody

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Data sheet of SRC (phospho Y215) polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityHuman
Host speciesRabbit
ApplicationsWB-Ce

More info about SRC (phospho Y215) polyclonal antibody

Brand: Abnova
Reference: PAB0612
Product name: SRC (phospho Y215) polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of SRC.
Gene id: 6714
Gene name: SRC
Gene alias: ASV|SRC1|c-SRC|p60-Src
Gene description: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Immunogen: Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y215 of human SRC.
Protein accession: NP_005408;P12931
Form: Liquid
Recommend dilutions: Western Blot (1:1000)
The optimal working dilution should be determined by the end user.
Storage buffer: In PBS (0.09% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Reactivity: Human
Application image: PAB0612-46-74-1.jpg
Application image note: FG Pancreatic Carcinoma Cell stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x).
SRC (phospho Y215) polyclonal antibody (Cat # PAB0612) was diluted in 1% BSA/TBST and incubated Overnight at 4 °C.
After washing 3x 5 min. with TBST the blots were incubated with Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at room temperature.
The blots were again washed 3x 5 min. with TBST and developed using ECL reagent.
Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD
Applications: WB-Ce
Shipping condition: Dry Ice
Publications: Activation of beta-catenin-TCF-mediated transcription by non-receptor tyrosine kinase v-Src.Haraguchi K, Nishida A, Ishidate T, Akiyama T.
Biochem Biophys Res Commun. 2004 Jan 23;313(4):841-4.

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