Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Human |
Host species | Rabbit |
Applications | WB-Ce |
Brand: | Abnova |
Reference: | PAB0612 |
Product name: | SRC (phospho Y215) polyclonal antibody |
Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of SRC. |
Gene id: | 6714 |
Gene name: | SRC |
Gene alias: | ASV|SRC1|c-SRC|p60-Src |
Gene description: | v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) |
Immunogen: | Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y215 of human SRC. |
Protein accession: | NP_005408;P12931 |
Form: | Liquid |
Recommend dilutions: | Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
Storage buffer: | In PBS (0.09% sodium azide) |
Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Product type: | Primary antibodies |
Host species: | Rabbit |
Antigen species / target species: | Human |
Reactivity: | Human |
Application image: | ![]() |
Application image note: | FG Pancreatic Carcinoma Cell stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x). SRC (phospho Y215) polyclonal antibody (Cat # PAB0612) was diluted in 1% BSA/TBST and incubated Overnight at 4 °C. After washing 3x 5 min. with TBST the blots were incubated with Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at room temperature. The blots were again washed 3x 5 min. with TBST and developed using ECL reagent. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD |
Applications: | WB-Ce |
Shipping condition: | Dry Ice |
Publications: | Activation of beta-catenin-TCF-mediated transcription by non-receptor tyrosine kinase v-Src.Haraguchi K, Nishida A, Ishidate T, Akiyama T. Biochem Biophys Res Commun. 2004 Jan 23;313(4):841-4. |