Reference: | P5275 |
Product name: | Collagenase Type 4 (C. histolyticum) |
Product description: | Lyophilized. Dialyzed. Contains lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity. |
Form: | Lyophilized |
Preparation method: | Native protein purified from Clostridium histolyticum |
Recommend dilutions: | Collagenase Assay; Pancreatic islet primary isolation. |
Storage instruction: | Store at 4°C on dry atmosphere. For primary cell isolation, culture and tissue dissociation: Reconstituted in balanced salt solutions (such as Hank's, Earle's and others) at concentrations from 0.05% to 0.5% (w/v). For enzymetic assay: Reconstituted in 0.05 M TES buffer with 0.36 mM calcium chloride, pH 7.5 at a concentration of 1.0 mg/ml. |
Tag: | None |
Shipping condition: | Blue Ice |