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Data sheet of His tag monoclonal antibody, clone HIS.H8
| Brand | Abnova |
| Product type | Primary antibodies |
| Host species | Mouse |
| Applications | IP,ELISA,IHC,Dot,IF,WB-Tr |
More info about His tag monoclonal antibody, clone HIS.H8
| Brand: | Abnova |
| Reference: | MAB9752 |
| Product name: | His tag monoclonal antibody, clone HIS.H8 |
| Product description: | Mouse monoclonal antibody raised against synthetic peptide of His tag. |
| Clone: | HIS.H8 |
| Isotype: | IgG2b |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to His tag. |
| Form: | Liquid |
| Concentration: | 1 mg/mL |
| Recommend dilutions: | Western Blot (1:1000-1:3000) Immunohistochemistry (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 10 mM PBS, pH 7.2 (0.05% sodium azide) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Specificity: | Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags). |
| Application image: |  |
| Application image note: | Immunofluorescence staining of HEK293 cells. A: untransfected control. B: His tag monoclonal antibody, clone HIS.H8 (Cat # MAB9752) (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue). |
| Applications: | IP,ELISA,IHC,Dot,IF,WB-Tr |
| Shipping condition: | Dry Ice |