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Data sheet of GDF15 monoclonal antibody, clone 23B3.D2.H5
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Mouse |
| Applications | WB,ELISA |
More info about GDF15 monoclonal antibody, clone 23B3.D2.H5
| Brand: | Abnova |
| Reference: | MAB8161 |
| Product name: | GDF15 monoclonal antibody, clone 23B3.D2.H5 |
| Product description: | Mouse monoclonal antibody raised against synthetic peptide of GDF15 . |
| Clone: | 23B3.D2.H5 |
| Isotype: | IgG1 |
| Gene id: | 9518 |
| Gene name: | GDF15 |
| Gene alias: | GDF-15|MIC-1|MIC1|NAG-1|PDF|PLAB|PTGFB |
| Gene description: | growth differentiation factor 15 |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to C-terminus end of human GDF15 . |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:2000) Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Western blot using GDF15 monoclonal antibody, clone 23B3D2.H5 (Cat # MAB8161). The blot shows detection of recombinant GDF15 protein present in Pichia pastoris whole cell lysates. Lane 1, yeast cell lysate expressing GDF15 H variant with SUMO expression tag at 36 KDa. Lane 2, yeast cell lysate expressing GDF15 D variant with SUMO expression tag at 36 KDa. Lane 3, yeast cell lysate expressing GDF15 H variant. Lane 4, yeast cell lysate expressing GDF15 D variant. Recombinant GDF15 proteins without SUMO correspond to monomer (15 KDa) and dimer (30 KDa) bands as indicated by the arrowheads. All lysates were run under reducing conditions. Primary antibody was used at a 1 : 1,000 dilution in TBS containg 1% BSA and 0.2% Tween, and reacted overnight at 4°C. For detection, a 1 : 40,000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody was used in Blocking Buffer for Fluorescent Western Blotting for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc™ 4000MP Imaging System. |
| Applications: | WB,ELISA |
| Shipping condition: | Dry Ice |
| Publications: | H6D polymorphism in macrophage-inhibitory cytokine-1 gene associated with prostate cancer.Lindmark F, Zheng SL, Wiklund F, Bensen J, Balter KA, Chang B, Hedelin M, Clark J, Stattin P, Meyers DA, Adami HO, Isaacs W, Gronberg H, Xu J. J Natl Cancer Inst. 2004 Aug 18;96(16):1248-54. |