Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Mouse |
Host species | Rat |
Applications | WB,IP,IHC-Fr,ICC,IHC-P,IF,Flow Cyt |
Brand: | Abnova |
Reference: | MAB7100 |
Product name: | Cd34 monoclonal antibody, clone MEC 14.7 |
Product description: | Rat monoclonal antibody raised against Cd34. |
Clone: | MEC 14.7 |
Isotype: | IgG2a |
Gene id: | 12490 |
Gene name: | Cd34 |
Gene alias: | AU040960 |
Gene description: | CD34 antigen |
Immunogen: | Full length mouse Cd34. |
Form: | Liquid |
Recommend dilutions: | Western Blot (1:250) Flow Cytometry (1 ug per million cells) Immunocytochemistry/Immunofluorescence (1:100-1:1000) Immunohistochemistry (1:250) Immunoprecipitation (1:10-1:500) The optimal working dilution should be determined by the end us |
Storage buffer: | In PBS (0.02% sodium azide) |
Storage instruction: | Store at 4°C for short term. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Product type: | Primary antibodies |
Host species: | Rat |
Antigen species / target species: | Mouse |
Specificity: | The reacts with Mouse CD34, a protein present on endothelial cells and hematopoietic progenitor cells. The recognizes a neuraminidase sensitive epitope on endothelium in vivo, particularly on small vessels and neoformed capillaries. |
Reactivity: | Mouse |
Application image: | ![]() |
Application image note: | Immunocytochemistry with Cd34 monoclonal antibody, clone MEC 14.7 (Cat # MAB7100). Localization of Cd34 expression, LRCs, and K15 expression in murine hair follicles. (A) Cd34 expression in formalin-fixed, paraffin-embedded dorsal skin from an untreated Tg.AC mouse, showing membrane localization in the hair follicle. Arrow indicates Cd34-positive cells. (B) Cd34 expression in a murine telogen hair follicle. (C)Cd34 expression in a murine anagen hair follicle. Arrow indicates Cd34-positive keratinocytes in panels B and C. Scale bars: 100 mum. |
Applications: | WB,IP,IHC-Fr,ICC,IHC-P,IF,Flow Cyt |
Shipping condition: | Dry Ice |
Publications: | Enrichment for living murine keratinocytes from the hair follicle bulge with the cell surface marker CD34.Trempus CS, Morris RJ, Bortner CD, Cotsarelis G, Faircloth RS, Reece JM, Tennant RW. J Invest Dermatol. 2003 Apr;120(4):501-11. |