Cd34 monoclonal antibody, clone MEC 14.7 View larger

Cd34 monoclonal antibody, clone MEC 14.7

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Data sheet of Cd34 monoclonal antibody, clone MEC 14.7

BrandAbnova
Product typePrimary antibodies
ReactivityMouse
Host speciesRat
ApplicationsWB,IP,IHC-Fr,ICC,IHC-P,IF,Flow Cyt

More info about Cd34 monoclonal antibody, clone MEC 14.7

Brand: Abnova
Reference: MAB7100
Product name: Cd34 monoclonal antibody, clone MEC 14.7
Product description: Rat monoclonal antibody raised against Cd34.
Clone: MEC 14.7
Isotype: IgG2a
Gene id: 12490
Gene name: Cd34
Gene alias: AU040960
Gene description: CD34 antigen
Immunogen: Full length mouse Cd34.
Form: Liquid
Recommend dilutions: Western Blot (1:250)
Flow Cytometry (1 ug per million cells)
Immunocytochemistry/Immunofluorescence (1:100-1:1000)
Immunohistochemistry (1:250)
Immunoprecipitation (1:10-1:500)
The optimal working dilution should be determined by the end us
Storage buffer: In PBS (0.02% sodium azide)
Storage instruction: Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rat
Antigen species / target species: Mouse
Specificity: The reacts with Mouse CD34, a protein present on endothelial cells and hematopoietic progenitor cells. The recognizes a neuraminidase sensitive epitope on endothelium in vivo, particularly on small vessels and neoformed capillaries.
Reactivity: Mouse
Application image: MAB7100-48-217-1.jpg
Application image note: Immunocytochemistry with Cd34 monoclonal antibody, clone MEC 14.7 (Cat # MAB7100).
Localization of Cd34 expression, LRCs, and K15 expression in murine hair follicles.
(A) Cd34 expression in formalin-fixed, paraffin-embedded dorsal skin from an untreated Tg.AC mouse, showing membrane localization in the hair follicle. Arrow indicates Cd34-positive cells.
(B) Cd34 expression in a murine telogen hair follicle.
(C)Cd34 expression in a murine anagen hair follicle. Arrow indicates Cd34-positive keratinocytes in panels B and C.
Scale bars: 100 mum.
Applications: WB,IP,IHC-Fr,ICC,IHC-P,IF,Flow Cyt
Shipping condition: Dry Ice
Publications: Enrichment for living murine keratinocytes from the hair follicle bulge with the cell surface marker CD34.Trempus CS, Morris RJ, Bortner CD, Cotsarelis G, Faircloth RS, Reece JM, Tennant RW.
J Invest Dermatol. 2003 Apr;120(4):501-11.

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