Brand: | Abnova |
Reference: | MAB6952 |
Product name: | PECAM1 monoclonal antibody, clone 158-2B3 |
Product description: | Mouse monoclonal antibody raised against native PECAM1. |
Clone: | 158-2B3 |
Isotype: | IgG1 |
Gene id: | 5175 |
Gene name: | PECAM1 |
Gene alias: | CD31|PECAM-1 |
Gene description: | platelet/endothelial cell adhesion molecule |
Immunogen: | Native purified PECAM1 from stimulated human leukocytes. |
Form: | Liquid |
Recommend dilutions: | The optimal working dilution should be determined by the end user. |
Storage buffer: | In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate) |
Storage instruction: | Store at 4°C. |
Product type: | Primary antibodies |
Host species: | Mouse |
Antigen species / target species: | Human |
Reactivity: | Human |
Application image: |  |
Application image note: | 5 X 105 cultured human Jurkat cells were incubated 45 minutes on ice with 80 ml of PECAM1 monoclonal antibody, clone 158-2B3 (Cat # MAB6952) at 10 mg/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 1.62 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. |
Applications: | Flow Cyt |
Shipping condition: | Blue Ice |
Publications: | The effect of intravenous administration of a chimeric anti-IgE antibody on serum IgE levels in atopic subjects: efficacy, safety, and pharmacokinetics.Corne J, Djukanovic R, Thomas L, Warner J, Botta L, Grandordy B, Gygax D, Heusser C, Patalano F, Richardson W, Kilchherr E, Staehelin T, Davis F, Gordon W, Sun L, Liou R, Wang G, Chang TW, Holgate S. J Clin Invest. 1997 Mar 1;99(5):879-87. |