Brand: | Abnova |
Reference: | MAB6947 |
Product name: | KLRD1 monoclonal antibody, clone HP-3D9 |
Product description: | Mouse monoclonal antibody raised against native KLRD1. |
Clone: | HP-3D9 |
Isotype: | IgG1 |
Gene id: | 3824 |
Gene name: | KLRD1 |
Gene alias: | CD94 |
Gene description: | killer cell lectin-like receptor subfamily D, member 1 |
Immunogen: | Native purified KLRD1 from cultured human NK cells . |
Form: | Liquid |
Recommend dilutions: | The optimal working dilution should be determined by the end user. |
Storage buffer: | In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate) |
Storage instruction: | Store at 4°C. |
Product type: | Primary antibodies |
Host species: | Mouse |
Antigen species / target species: | Human |
Reactivity: | Human |
Application image: |  |
Application image note: | Ficoll prepared human peripheral white blood cells were stimulated by incubating 1 day at 5 x 106 cells/mL in RPMI 10% FBS media including 5 ug/mL Phytohemagglutinin-P. 5 X 105 cells per tube were then washed and incubated 45 minutes on ice with 80 uL of KLRD1 monoclonal antibody, clone HP-3D9 (Cat # MAB6947) at a concentration of 20 ug/mL. Cells were washed twice and incubated with 2oreagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 40% population of the cells stained positive with a mean shift of 1.10 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. |
Applications: | Flow Cyt |
Shipping condition: | Blue Ice |
Publications: | A novel functional cell surface dimer (Kp43) expressed by natural killer cells and T cell receptor-gamma/delta+ T lymphocytes. I. Inhibition of the IL-2-dependent proliferation by anti-Kp43 monoclonal antibody.Aramburu J, Balboa MA, Ramirez A, Silva A, Acevedo A, Sanchez-Madrid F, De Landazuri MO, Lopez-Botet M. J Immunol. 1990 Apr 15;144(8):3238-47. |