Brand: | Abnova |
Reference: | MAB6945 |
Product name: | ITGB2 monoclonal antibody, clone IB4 |
Product description: | Mouse monoclonal antibody raised against native ITGB2. |
Clone: | IB4 |
Isotype: | IgG2a |
Gene id: | 3689 |
Gene name: | ITGB2 |
Gene alias: | CD18|LAD|LCAMB|LFA-1|MAC-1|MF17|MFI7 |
Gene description: | integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) |
Immunogen: | Native purified ITGB2 from human peripheral blood monocytes. |
Form: | Liquid |
Recommend dilutions: | The optimal working dilution should be determined by the end user. |
Storage buffer: | In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate) |
Storage instruction: | Store at 4°C. |
Product type: | Primary antibodies |
Host species: | Mouse |
Antigen species / target species: | Human |
Reactivity: | Human |
Application image: |  |
Application image note: | 5 X 105 cultured human HPB-MLT cells per tube were incubated 45 minutes on ice with 80 uL of ITGB2 monoclonal antibody, clone IB4 (Cat # MAB6945) at a concentration of 5 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive (67% net) with a mean shift of 0.92 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. |
Applications: | Flow Cyt |
Shipping condition: | Blue Ice |
Publications: | Involvement of the "I" domain of LFA-1 in selective binding to ligands ICAM-1 and ICAM-3.Landis RC, McDowall A, Holness CL, Littler AJ, Simmons DL, Hogg N. J Cell Biol. 1994 Jul;126(2):529-37. |