ITGB2 monoclonal antibody, clone IB4 View larger

ITGB2 monoclonal antibody, clone IB4

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Data sheet of ITGB2 monoclonal antibody, clone IB4

BrandAbnova
Product typePrimary antibodies
ReactivityHuman
Host speciesMouse
ApplicationsFlow Cyt

More info about ITGB2 monoclonal antibody, clone IB4

Brand: Abnova
Reference: MAB6945
Product name: ITGB2 monoclonal antibody, clone IB4
Product description: Mouse monoclonal antibody raised against native ITGB2.
Clone: IB4
Isotype: IgG2a
Gene id: 3689
Gene name: ITGB2
Gene alias: CD18|LAD|LCAMB|LFA-1|MAC-1|MF17|MFI7
Gene description: integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
Immunogen: Native purified ITGB2 from human peripheral blood monocytes.
Form: Liquid
Recommend dilutions: The optimal working dilution should be determined by the end user.
Storage buffer: In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate)
Storage instruction: Store at 4°C.
Product type: Primary antibodies
Host species: Mouse
Antigen species / target species: Human
Reactivity: Human
Application image: MAB6945-84-277-1.jpg
Application image note: 5 X 105 cultured human HPB-MLT cells per tube were incubated 45 minutes on ice with 80 uL of ITGB2 monoclonal antibody, clone IB4 (Cat # MAB6945) at a concentration of 5 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive (67% net) with a mean shift of 0.92 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration.
Applications: Flow Cyt
Shipping condition: Blue Ice
Publications: Involvement of the "I" domain of LFA-1 in selective binding to ligands ICAM-1 and ICAM-3.Landis RC, McDowall A, Holness CL, Littler AJ, Simmons DL, Hogg N.
J Cell Biol. 1994 Jul;126(2):529-37.

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