Brand: | Abnova |
Reference: | MAB6942 |
Product name: | ITGAL monoclonal antibody, clone 38 |
Product description: | Mouse monoclonal antibody raised against native ITGAL. |
Clone: | 38 |
Isotype: | IgG2a |
Gene id: | 3683 |
Gene name: | ITGAL |
Gene alias: | CD11A|LFA-1|LFA1A |
Gene description: | integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) |
Immunogen: | Native purified ITGAL from human monocytes. |
Form: | Liquid |
Recommend dilutions: | The optimal working dilution should be determined by the end user. |
Storage buffer: | In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate) |
Storage instruction: | Store at 4°C. |
Product type: | Primary antibodies |
Host species: | Mouse |
Antigen species / target species: | Human |
Reactivity: | Human |
Application image: |  |
Application image note: | 5 X 105 cultured HPB-MLT human tumor cells were washed and incubated 45 minutes on ice with 80 uL of ITGAL monoclonal antibody, clone 38 (Cat # MAB6942) at 5 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.28 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. |
Applications: | Flow Cyt |
Shipping condition: | Blue Ice |
Publications: | Regulated expression of Mg2+ binding epitope on leukocyte integrin alpha subunits.Dransfield I, Hogg N. EMBO J. 1989 Dec 1;8(12):3759-65. |