ICAM3 monoclonal antibody, clone 186-2G9 View larger

ICAM3 monoclonal antibody, clone 186-2G9

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Data sheet of ICAM3 monoclonal antibody, clone 186-2G9

BrandAbnova
Product typePrimary antibodies
ReactivityHuman
Host speciesMouse
ApplicationsFlow Cyt

More info about ICAM3 monoclonal antibody, clone 186-2G9

Brand: Abnova
Reference: MAB6936
Product name: ICAM3 monoclonal antibody, clone 186-2G9
Product description: Mouse monoclonal antibody raised against native ICAM3.
Clone: 186-2G9
Isotype: IgG2b, kappa
Gene id: 3385
Gene name: ICAM3
Gene alias: CD50|CDW50|ICAM-R
Gene description: intercellular adhesion molecule 3
Immunogen: Native purified ICAM3 from stimulated human leukocytes.
Form: Liquid
Recommend dilutions: The optimal working dilution should be determined by the end user.
Storage buffer: In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate)
Storage instruction: Store at 4°C.
Product type: Primary antibodies
Host species: Mouse
Antigen species / target species: Human
Reactivity: Human
Application image: MAB6936-84-6-1.jpg
Application image note: 5 X 105 cultured human Jurkat cells per tube were washed and preincubated ~5 minutes on ice with 20 ml of 250 mg/mL human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ml of ICAM3 monoclonal antibody, clone 186-2G9 (Cat # MAB6936) at 10 mg/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 1.84 log10 fluorescent units when compared to a Mouse IgG2b negative control at a similar concentration.
Applications: Flow Cyt
Shipping condition: Blue Ice
Publications: Analysis of the binding site on intercellular adhesion molecule 3 for the leukocyte integrin lymphocyte function-associated antigen 1.Holness CL, Bates PA, Little AJ, Buckley CD, McDowall A, Bossy D, Hogg N, Simmons DL.
J Biol Chem. 1995 Jan 13;270(2):877-84.

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