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Data sheet of ICAM3 monoclonal antibody, clone 186-2G9
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Mouse |
| Applications | Flow Cyt |
More info about ICAM3 monoclonal antibody, clone 186-2G9
| Brand: | Abnova |
| Reference: | MAB6936 |
| Product name: | ICAM3 monoclonal antibody, clone 186-2G9 |
| Product description: | Mouse monoclonal antibody raised against native ICAM3. |
| Clone: | 186-2G9 |
| Isotype: | IgG2b, kappa |
| Gene id: | 3385 |
| Gene name: | ICAM3 |
| Gene alias: | CD50|CDW50|ICAM-R |
| Gene description: | intercellular adhesion molecule 3 |
| Immunogen: | Native purified ICAM3 from stimulated human leukocytes. |
| Form: | Liquid |
| Recommend dilutions: | The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate) |
| Storage instruction: | Store at 4°C. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | 5 X 105 cultured human Jurkat cells per tube were washed and preincubated ~5 minutes on ice with 20 ml of 250 mg/mL human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ml of ICAM3 monoclonal antibody, clone 186-2G9 (Cat # MAB6936) at 10 mg/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 1.84 log10 fluorescent units when compared to a Mouse IgG2b negative control at a similar concentration. |
| Applications: | Flow Cyt |
| Shipping condition: | Blue Ice |
| Publications: | Analysis of the binding site on intercellular adhesion molecule 3 for the leukocyte integrin lymphocyte function-associated antigen 1.Holness CL, Bates PA, Little AJ, Buckley CD, McDowall A, Bossy D, Hogg N, Simmons DL. J Biol Chem. 1995 Jan 13;270(2):877-84. |