MHC Class I monoclonal antibody, clone 3F10 View larger

MHC Class I monoclonal antibody, clone 3F10

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Data sheet of MHC Class I monoclonal antibody, clone 3F10

BrandAbnova
Product typePrimary antibodies
ReactivityHuman
Host speciesMouse
ApplicationsFlow Cyt

More info about MHC Class I monoclonal antibody, clone 3F10

Brand: Abnova
Reference: MAB6933
Product name: MHC Class I monoclonal antibody, clone 3F10
Product description: Mouse monoclonal antibody raised against native MHC Class I.
Clone: 3F10
Isotype: IgG2a
Immunogen: Native purified MHC Class I from human HSB-2 T cell line.
Form: Liquid
Recommend dilutions: The optimal working dilution should be determined by the end user.
Storage buffer: In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate)
Storage instruction: Store at 4°C.
Product type: Primary antibodies
Host species: Mouse
Antigen species / target species: Human
Reactivity: Human
Application image: MAB6933-84-277-1.jpg
Application image note: 5 X 105 cultured HPB-MLT cells were washed and incubated 45 minutes on ice with 80 uL of anti-MHC Class I (FITC) at a 1 : 50 dilution. Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.77 log10 fluorescent units when compared to a Mouse IgG2a/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 uL of 0.5 mg/mL MHC Class I monoclonal antibody, clone 3F10 (Cat # MAB6933).
Applications: Flow Cyt
Shipping condition: Blue Ice
Publications: Structure, function, and diversity of class I major histocompatibility complex molecules.Bjorkman PJ, Parham P.
Annu Rev Biochem. 1990;59:253-88.

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