Brand: | Abnova |
Reference: | MAB6932 |
Product name: | GYPA monoclonal antibody, clone A63-B/C2 |
Product description: | Mouse monoclonal antibody raised against native GYPA. |
Clone: | A63-B/C2 |
Isotype: | IgM |
Gene id: | 2993 |
Gene name: | GYPA |
Gene alias: | CD235a|GPA|GPErik|GPSAT|GpMiIII|HGpMiIII|HGpMiV|HGpMiX|HGpMiXI|HGpSta(C)|MN|MNS |
Gene description: | glycophorin A (MNS blood group) |
Immunogen: | Native purified GYPA from neuraminidase treated human erythrocytes. |
Form: | Liquid |
Recommend dilutions: | The optimal working dilution should be determined by the end user. |
Storage buffer: | In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate) |
Storage instruction: | Store at 4°C. |
Product type: | Primary antibodies |
Host species: | Mouse |
Antigen species / target species: | Human |
Reactivity: | Human |
Application image: |  |
Application image note: | 107 human red blood cells were washed and preincubated 5 minutes with 20 uL of 250 ug/mL human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80ul of anti-CD235a (Glycophorin A) /Biotin at 20 ug/mL. Cells were washed twice and incubated with Streptavidin/R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.27 log10 fluorescent units when compared to a Mouse IgM/Biotin negative control at a similar concentration. Binding was 82% blocked when pre incubated 10 minutes with 20 uL of 0.5 mg/mL GYPA monoclonal antibody, clone A63-B/C2 (Cat # MAB6932). |
Applications: | Flow Cyt |
Shipping condition: | Blue Ice |
Publications: | The expression of human blood group antigens during erythropoiesis in a cell culture system.Southcott MJ, Tanner MJ, Anstee DJ. Blood. 1999 Jun 15;93(12):4425-35. |