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Data sheet of GYPA monoclonal antibody, clone A63-B/C2
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Mouse |
| Applications | Flow Cyt |
More info about GYPA monoclonal antibody, clone A63-B/C2
| Brand: | Abnova |
| Reference: | MAB6932 |
| Product name: | GYPA monoclonal antibody, clone A63-B/C2 |
| Product description: | Mouse monoclonal antibody raised against native GYPA. |
| Clone: | A63-B/C2 |
| Isotype: | IgM |
| Gene id: | 2993 |
| Gene name: | GYPA |
| Gene alias: | CD235a|GPA|GPErik|GPSAT|GpMiIII|HGpMiIII|HGpMiV|HGpMiX|HGpMiXI|HGpSta(C)|MN|MNS |
| Gene description: | glycophorin A (MNS blood group) |
| Immunogen: | Native purified GYPA from neuraminidase treated human erythrocytes. |
| Form: | Liquid |
| Recommend dilutions: | The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate) |
| Storage instruction: | Store at 4°C. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | 107 human red blood cells were washed and preincubated 5 minutes with 20 uL of 250 ug/mL human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80ul of anti-CD235a (Glycophorin A) /Biotin at 20 ug/mL. Cells were washed twice and incubated with Streptavidin/R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.27 log10 fluorescent units when compared to a Mouse IgM/Biotin negative control at a similar concentration. Binding was 82% blocked when pre incubated 10 minutes with 20 uL of 0.5 mg/mL GYPA monoclonal antibody, clone A63-B/C2 (Cat # MAB6932). |
| Applications: | Flow Cyt |
| Shipping condition: | Blue Ice |
| Publications: | The expression of human blood group antigens during erythropoiesis in a cell culture system.Southcott MJ, Tanner MJ, Anstee DJ. Blood. 1999 Jun 15;93(12):4425-35. |