GYPA monoclonal antibody, clone A63-B/C2 View larger

GYPA monoclonal antibody, clone A63-B/C2

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Data sheet of GYPA monoclonal antibody, clone A63-B/C2

BrandAbnova
Product typePrimary antibodies
ReactivityHuman
Host speciesMouse
ApplicationsFlow Cyt

More info about GYPA monoclonal antibody, clone A63-B/C2

Brand: Abnova
Reference: MAB6932
Product name: GYPA monoclonal antibody, clone A63-B/C2
Product description: Mouse monoclonal antibody raised against native GYPA.
Clone: A63-B/C2
Isotype: IgM
Gene id: 2993
Gene name: GYPA
Gene alias: CD235a|GPA|GPErik|GPSAT|GpMiIII|HGpMiIII|HGpMiV|HGpMiX|HGpMiXI|HGpSta(C)|MN|MNS
Gene description: glycophorin A (MNS blood group)
Immunogen: Native purified GYPA from neuraminidase treated human erythrocytes.
Form: Liquid
Recommend dilutions: The optimal working dilution should be determined by the end user.
Storage buffer: In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate)
Storage instruction: Store at 4°C.
Product type: Primary antibodies
Host species: Mouse
Antigen species / target species: Human
Reactivity: Human
Application image: MAB6932-84-106-1.jpg
Application image note: 107 human red blood cells were washed and preincubated 5 minutes with 20 uL of 250 ug/mL human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80ul of anti-CD235a (Glycophorin A) /Biotin at 20 ug/mL. Cells were washed twice and incubated with Streptavidin/R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.27 log10 fluorescent units when compared to a Mouse IgM/Biotin negative control at a similar concentration. Binding was 82% blocked when pre incubated 10 minutes with 20 uL of 0.5 mg/mL GYPA monoclonal antibody, clone A63-B/C2 (Cat # MAB6932).
Applications: Flow Cyt
Shipping condition: Blue Ice
Publications: The expression of human blood group antigens during erythropoiesis in a cell culture system.Southcott MJ, Tanner MJ, Anstee DJ.
Blood. 1999 Jun 15;93(12):4425-35.

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