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Data sheet of CD40 monoclonal antibody, clone EA-5
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Mouse |
| Applications | Flow Cyt |
More info about CD40 monoclonal antibody, clone EA-5
| Brand: | Abnova |
| Reference: | MAB6912 |
| Product name: | CD40 monoclonal antibody, clone EA-5 |
| Product description: | Mouse monoclonal antibody raised against native CD40. |
| Clone: | EA-5 |
| Isotype: | IgG1 |
| Gene id: | 958 |
| Gene name: | CD40 |
| Gene alias: | Bp50|CDW40|MGC9013|TNFRSF5|p50 |
| Gene description: | CD40 molecule, TNF receptor superfamily member 5 |
| Immunogen: | Native purified CD40 from murine L cells. |
| Form: | Liquid |
| Recommend dilutions: | The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate) |
| Storage instruction: | Store at 4°C. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | 5 X 105 cultured Raji human tumor cells were incubated 45 minutes on ice with 80 uL of CD40 monoclonal antibody, clone EA-5 (Cat # MAB6912) at 5 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.64 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. |
| Applications: | Flow Cyt |
| Shipping condition: | Blue Ice |
| Publications: | Soluble forms of CD40 inhibit biologic responses of human B cells.Fanslow WC, Anderson DM, Grabstein KH, Clark EA, Cosman D, Armitage RJ. J Immunol. 1992 Jul 15;149(2):655-60. |