Brand: | Abnova |
Reference: | MAB6912 |
Product name: | CD40 monoclonal antibody, clone EA-5 |
Product description: | Mouse monoclonal antibody raised against native CD40. |
Clone: | EA-5 |
Isotype: | IgG1 |
Gene id: | 958 |
Gene name: | CD40 |
Gene alias: | Bp50|CDW40|MGC9013|TNFRSF5|p50 |
Gene description: | CD40 molecule, TNF receptor superfamily member 5 |
Immunogen: | Native purified CD40 from murine L cells. |
Form: | Liquid |
Recommend dilutions: | The optimal working dilution should be determined by the end user. |
Storage buffer: | In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate) |
Storage instruction: | Store at 4°C. |
Product type: | Primary antibodies |
Host species: | Mouse |
Antigen species / target species: | Human |
Reactivity: | Human |
Application image: |  |
Application image note: | 5 X 105 cultured Raji human tumor cells were incubated 45 minutes on ice with 80 uL of CD40 monoclonal antibody, clone EA-5 (Cat # MAB6912) at 5 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.64 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. |
Applications: | Flow Cyt |
Shipping condition: | Blue Ice |
Publications: | Soluble forms of CD40 inhibit biologic responses of human B cells.Fanslow WC, Anderson DM, Grabstein KH, Clark EA, Cosman D, Armitage RJ. J Immunol. 1992 Jul 15;149(2):655-60. |