Brand: | Abnova |
Reference: | MAB6908 |
Product name: | CD36 monoclonal antibody, clone SMO |
Product description: | Mouse monoclonal antibody raised against native CD36. |
Clone: | SMO |
Isotype: | IgM |
Gene id: | 948 |
Gene name: | CD36 |
Gene alias: | CHDS7|FAT|GP3B|GP4|GPIV|PASIV|SCARB3 |
Gene description: | CD36 molecule (thrombospondin receptor) |
Immunogen: | Native purified CD36 from human tonsil cells/PBM. |
Form: | Liquid |
Recommend dilutions: | The optimal working dilution should be determined by the end user. |
Storage buffer: | In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate) |
Storage instruction: | Store at 4°C. Do not freeze. |
Product type: | Primary antibodies |
Host species: | Mouse |
Antigen species / target species: | Human |
Reactivity: | Human |
Application image: |  |
Application image note: | 5 X 105 ficoll prepared human peripheral blood lymphocytes were washed and preincubated 5 minutes with 20 m l of 250 mg/mL human IgG (To block non specific binding) after which they were incubated 45 minutes on ice with 80m l of CD36 monoclonal antibody, clone SMO (Cat # MAB6908) at a concentration of 5 mg/mL. Cells were washed twice and incubated with Goat anti-Mouse Ig/FITC, after which they were washed three times, fixed and analyzed using a BD FACstar plus. A net 16% sub population of the cells stained positive with a mean shift of 1.05 log10 fluorescent units when compared to a Mouse IgM negative control at a similar concentration. |
Applications: | Flow Cyt |
Shipping condition: | Blue Ice |
Publications: | Monoclonal antibodies specific for human monocytes, granulocytes and endothelium.Hogg N, MacDonald S, Slusarenko M, Beverley PC. Immunology. 1984 Dec;53(4):753-67. |