Brand: | Abnova |
Reference: | MAB6903 |
Product name: | CD27 monoclonal antibody, clone M-T271 |
Product description: | Mouse monoclonal antibody raised against native CD27. |
Clone: | M-T271 |
Isotype: | IgG1 |
Gene id: | 939 |
Gene name: | CD27 |
Gene alias: | MGC20393|S152|T14|TNFRSF7|Tp55 |
Gene description: | CD27 molecule |
Immunogen: | Native purified CD27 from T cell line (from patient with CLL). |
Form: | Liquid |
Recommend dilutions: | The optimal working dilution should be determined by the end user. |
Storage buffer: | In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate) |
Storage instruction: | Store at 4°C. |
Product type: | Primary antibodies |
Host species: | Mouse |
Antigen species / target species: | Human |
Reactivity: | Human |
Application image: |  |
Application image note: | 5 X 105 ficoll prepared human peripheral white blood cells were washed and preincubated 5 minutes with 20 uL of 250 ug/mL human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 uL of CD27 monoclonal antibody, clone M-T271 (Cat # MAB6903) at 10 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 64% sub population of the cells stained positive with a mean shift of 1.0 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. |
Applications: | Flow Cyt |
Shipping condition: | Blue Ice |
Publications: | CD27: marker and mediator of T-cell activation?Hintzen RQ, de Jong R, Lens SM, van Lier RA. Immunol Today. 1994 Jul;15(7):307-11. |