CD27 monoclonal antibody, clone M-T271 View larger

CD27 monoclonal antibody, clone M-T271

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Data sheet of CD27 monoclonal antibody, clone M-T271

BrandAbnova
Product typePrimary antibodies
ReactivityHuman
Host speciesMouse
ApplicationsFlow Cyt

More info about CD27 monoclonal antibody, clone M-T271

Brand: Abnova
Reference: MAB6903
Product name: CD27 monoclonal antibody, clone M-T271
Product description: Mouse monoclonal antibody raised against native CD27.
Clone: M-T271
Isotype: IgG1
Gene id: 939
Gene name: CD27
Gene alias: MGC20393|S152|T14|TNFRSF7|Tp55
Gene description: CD27 molecule
Immunogen: Native purified CD27 from T cell line (from patient with CLL).
Form: Liquid
Recommend dilutions: The optimal working dilution should be determined by the end user.
Storage buffer: In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate)
Storage instruction: Store at 4°C.
Product type: Primary antibodies
Host species: Mouse
Antigen species / target species: Human
Reactivity: Human
Application image: MAB6903-84-78-1.jpg
Application image note: 5 X 105 ficoll prepared human peripheral white blood cells were washed and preincubated 5 minutes with 20 uL of 250 ug/mL human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 uL of CD27 monoclonal antibody, clone M-T271 (Cat # MAB6903) at 10 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 64% sub population of the cells stained positive with a mean shift of 1.0 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
Applications: Flow Cyt
Shipping condition: Blue Ice
Publications: CD27: marker and mediator of T-cell activation?Hintzen RQ, de Jong R, Lens SM, van Lier RA.
Immunol Today. 1994 Jul;15(7):307-11.

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