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Data sheet of CD27 monoclonal antibody, clone M-T271
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Mouse |
| Applications | Flow Cyt |
More info about CD27 monoclonal antibody, clone M-T271
| Brand: | Abnova |
| Reference: | MAB6903 |
| Product name: | CD27 monoclonal antibody, clone M-T271 |
| Product description: | Mouse monoclonal antibody raised against native CD27. |
| Clone: | M-T271 |
| Isotype: | IgG1 |
| Gene id: | 939 |
| Gene name: | CD27 |
| Gene alias: | MGC20393|S152|T14|TNFRSF7|Tp55 |
| Gene description: | CD27 molecule |
| Immunogen: | Native purified CD27 from T cell line (from patient with CLL). |
| Form: | Liquid |
| Recommend dilutions: | The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate) |
| Storage instruction: | Store at 4°C. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | 5 X 105 ficoll prepared human peripheral white blood cells were washed and preincubated 5 minutes with 20 uL of 250 ug/mL human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 uL of CD27 monoclonal antibody, clone M-T271 (Cat # MAB6903) at 10 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 64% sub population of the cells stained positive with a mean shift of 1.0 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. |
| Applications: | Flow Cyt |
| Shipping condition: | Blue Ice |
| Publications: | CD27: marker and mediator of T-cell activation?Hintzen RQ, de Jong R, Lens SM, van Lier RA. Immunol Today. 1994 Jul;15(7):307-11. |