Brand: | Abnova |
Reference: | MAB6901 |
Product name: | CD19 monoclonal antibody, clone BU12 |
Product description: | Mouse monoclonal antibody raised against native CD19. |
Clone: | BU12 |
Isotype: | IgG1 |
Gene id: | 930 |
Gene name: | CD19 |
Gene alias: | B4|MGC12802 |
Gene description: | CD19 molecule |
Immunogen: | Native purified CD19 from human EB-4 Burkitt lymphoma cell line . |
Form: | Liquid |
Recommend dilutions: | The optimal working dilution should be determined by the end user. |
Storage buffer: | In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate) |
Storage instruction: | Store at 4°C. |
Product type: | Primary antibodies |
Host species: | Mouse |
Antigen species / target species: | Human |
Reactivity: | Human |
Application image: |  |
Application image note: | 5 X 105 cultured Raji cells were washed and incubated 45 minutes on ice with 80 uL of CD19 monoclonal antibody, clone BU12 (Cat # MAB6901) at 10 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed using a BD FACstar plus. Cells stained positive with a mean shift of 1.58 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. |
Applications: | Flow Cyt |
Shipping condition: | Blue Ice |
Publications: | The CD19/CR2/TAPA-1 complex of B lymphocytes: linking natural to acquired immunity.Fearon DT, Carter RH. Annu Rev Immunol. 1995;13:127-49. |