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Data sheet of 5-Methylcytosine monoclonal antibody, clone 5MC-CD
| Brand | Abnova |
| Product type | Primary antibodies |
| Host species | Mouse |
| Applications | ICC,IF |
More info about 5-Methylcytosine monoclonal antibody, clone 5MC-CD
| Brand: | Abnova |
| Reference: | MAB6766 |
| Product name: | 5-Methylcytosine monoclonal antibody, clone 5MC-CD |
| Product description: | Mouse monoclonal antibody raised against 5-Methylcytosine. |
| Clone: | 5MC-CD |
| Isotype: | IgM |
| Immunogen: | 5-Methylcytosine conjugated with BSA. |
| Form: | Liquid |
| Recommend dilutions: | Immunocytochemistry (1:50-1:100) Western Blot (~1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In antiserum (0.05% sodium azide) |
| Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Application image: |  |
| Application image note: | Detection of DNA methylation in mouse embryonic stem cells by immunofluorescence staining with the 5-Methylcytosine monoclonal antibody, clone 5MC-CD (Cat # MAB6766). Intense 5-methylcytosine staining at pericentromeric regions was seen in the mitotic chromosome and interphase nuclei of ESCs. |
| Applications: | ICC,IF |
| Shipping condition: | Dry Ice |
| Publications: | A chloroplast-resident DNA methyltransferase is responsible for hypermethylation of chloroplast genes in Chlamydomonas maternal gametes.Nishiyama R, Ito M, Yamaguchi Y, Koizumi N, Sano H. Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):5925-30. |