RB1 (phospho T821) monoclonal antibody, clone 24A7 View larger

RB1 (phospho T821) monoclonal antibody, clone 24A7

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Data sheet of RB1 (phospho T821) monoclonal antibody, clone 24A7

BrandAbnova
Product typePrimary antibodies
ReactivityHuman
Host speciesMouse
ApplicationsWB,ELISA

More info about RB1 (phospho T821) monoclonal antibody, clone 24A7

Brand: Abnova
Reference: MAB6765
Product name: RB1 (phospho T821) monoclonal antibody, clone 24A7
Product description: Mouse monoclonal antibody raised against synthetic phosphopeptide of RB1.
Clone: 24A7
Isotype: IgG1, kappa
Gene id: 5925
Gene name: RB1
Gene alias: OSRC|RB|p105-Rb|pRb|pp110
Gene description: retinoblastoma 1
Immunogen: Synthetic phosphopeptide corresponding to residues surrounding T821 of human RB1.
Form: Liquid
Recommend dilutions: Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.
Storage buffer: In antiserum (0.05% sodium azide)
Storage instruction: Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Mouse
Antigen species / target species: Human
Specificity: This antibody is specific to human RB1 phosphorylated at Thr821. It can detect endogenous levels of RB1 phosphorylated at Thr821 in most cell lines.
Reactivity: Human
Application image: MAB6765-12-92-1.jpg
Application image note: Specificity of the RB1 (phospho T821) monoclonal antibody, clone 24A7 (Cat # MAB6765) as demonstrated by western blotting.
Crude extracts of human lung carcinoma cell line H1299 transfected with plasmid expressing Myc-tagged wild-type Rb (Lane 1), Rb (S795A, lane 2) or Rb (T821A, lane 3) were immunoprecipitated with anti-Myc antibody and the precipitates were analyzed by western blotting with RB1 (phospho T821) monoclonal antibody, clone 24A7 (Cat # MAB6765).
Data provided by Dr. Y. Inoue at the JFCR Cancer Research Institute, Tokyo.
Applications: WB,ELISA
Shipping condition: Dry Ice
Publications: E2F: a link between the Rb tumor suppressor protein and viral oncoproteins.Nevins JR.
Science. 1992 Oct 16;258(5081):424-9.

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