Brand: | Abnova |
Reference: | MAB6208 |
Product name: | Hspa5 monoclonal antibody, clone 76-E6 |
Product description: | Rat monoclonal antibody raised against Hspa5. |
Clone: | 76-E6 |
Isotype: | IgG1 |
Gene id: | 14828 |
Gene name: | Hspa5 |
Gene alias: | 78kDa|AL022860|AU019543|Bip|D2Wsu141e|D2Wsu17e|Grp78|Hsce70|SEZ-7|Sez7|mBiP |
Gene description: | heat shock protein 5 |
Immunogen: | Mouse BiP:u chain complexes. |
Form: | Liquid |
Recommend dilutions: | The optimal working dilution should be determined by the end user. |
Storage buffer: | In 100 mM BBS, pH 8.2 |
Storage instruction: | Store at 4°C. |
Product type: | Primary antibodies |
Host species: | Rat |
Antigen species / target species: | Mouse |
Specificity: | BiP (Immunoglobulin heavy chain binding protein, Mr 78 KDa). |
Reactivity: | Mouse |
Application image: |  |
Application image note: | Recombinant hamster Hspa5 mutant (HCB1-, lane 1), full-length recombinant hamster Hspa5 (Lane 2) and total cell lysate from Ag8.653, a mouse myeloma cell line (Lane 3) were resolved by a 10% SDS-PAGE. The proteins were transferred onto a PVDF membrane and incubated with Hspa5 monoclonal antibody, clone 76-E6 (Biotin) followed by Streptavidin-HRP. The blot was then developed using ECL Western blot detection reagents (Amersham Life Science, Arlington Heights, IL). Note : We are grateful to Dr. Linda Hendershot for the recombinant hamster Hspa5 proteins and for helpful suggestions. |
Applications: | WB,ELISA |
Shipping condition: | Blue Ice |
Publications: | BiP (GRP78), an essential hsp70 resident protein in the endoplasmic reticulum.Haas IG. Experientia. 1994 Nov 30;50(11-12):1012-20. |