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Data sheet of Cd86 monoclonal antibody, clone GL1 (PE/Cy7)
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Mouse |
| Host species | Rat |
| Applications | IP,IHC-Fr,Flow Cyt |
More info about Cd86 monoclonal antibody, clone GL1 (PE/Cy7)
| Brand: | Abnova |
| Reference: | MAB5756 |
| Product name: | Cd86 monoclonal antibody, clone GL1 (PE/Cy7) |
| Product description: | Rat monoclonal antibody raised against native Cd86. |
| Clone: | GL1 |
| Isotype: | IgG2a, kappa |
| Gene id: | 12524 |
| Gene name: | Cd86 |
| Gene alias: | B7|B7-2|B7.2|B70|CLS1|Cd28l2|ETC-1|Ly-58|Ly58|MB7|MB7-2|TS/A-2 |
| Gene description: | CD86 antigen |
| Immunogen: | Native purified Cd86 from LPS-activated mouse B cells. |
| Form: | Liquid |
| Recommend dilutions: | Flow Cytometry (0.1 ug/106 cells) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.09% sodium azide) |
| Storage instruction: | Store in the dark at 4°C. Do not freeze. Avoid prolonged exposure to light. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Conjugate tag: | PE/Cy7 |
| Product type: | Primary antibodies |
| Host species: | Rat |
| Antigen species / target species: | Mouse |
| Specificity: | Mouse CD86, the B7-2 co-stimulatory molecule and a ligand for CD28 and CD152/CTLA-4. |
| Reactivity: | Mouse |
| Application image: |  |
| Application image note: | BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with Cd86 monoclonal antibody, clone GL1 (FITC) (Cat # MAB5751). A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScanâ„¢ flow cytometer (BDIS, San Jose, CA). |
| Applications: | IP,IHC-Fr,Flow Cyt |
| Shipping condition: | Blue Ice |
| Publications: | Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function.Hathcock KS, Laszlo G, Pucillo C, Linsley P, Hodes RJ. J Exp Med. 1994 Aug 1;180(2):631-40. |