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Data sheet of MPO monoclonal antibody, clone 2C7
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Mouse |
| Applications | WB,ELISA,IHC-Fr,IHC-P,Flow Cyt |
More info about MPO monoclonal antibody, clone 2C7
| Brand: | Abnova |
| Reference: | MAB5342 |
| Product name: | MPO monoclonal antibody, clone 2C7 |
| Product description: | Mouse monoclonal antibody raised against MPO. |
| Clone: | 2C7 |
| Isotype: | IgG1 |
| Gene id: | 4353 |
| Gene name: | MPO |
| Gene alias: | - |
| Gene description: | myeloperoxidase |
| Immunogen: | Human MPO. |
| Form: | Liquid |
| Recommend dilutions: | Immunohistochemistry (Frozen sections) (1:1000-1: 5000) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:1000) Flow Cytometry (1:50-1:100) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.09% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Antigen species / target species: | Human |
| Specificity: | This antibody recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55kD) and two light chains (15kD), the heavy chains being linked by a disulphide bond. MON 8074 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. This antibody also recognizes recombinant MPO in Western blots and weakly in ELISA. This antibody may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeablisation. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Immunohistochemistry staining of MPO in human bone marrow (formalin-fixed paraffin-embedded) using MPO monoclonal antibody, clone 2C7 (Cat. # MAB5342). |
| Applications: | WB,ELISA,IHC-Fr,IHC-P,Flow Cyt |
| Shipping condition: | Dry Ice |
| Publications: | Human Neutrophils Use Different Mechanisms To Kill Aspergillus fumigatus Conidia and Hyphae: Evidence from Phagocyte Defects.Gazendam RP, van Hamme JL, Tool AT, Hoogenboezem M, van den Berg JM, Prins JM, Vitkov L, van de Veerdonk FL, van den Berg TK, Roos D, Kuijpers TW. J Immunol. 2015 Dec 30. [Epub ahead of print] |