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Data sheet of IL2RA monoclonal antibody, clone MEM-181 (FITC)
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Mouse |
| Applications | Flow Cyt |
More info about IL2RA monoclonal antibody, clone MEM-181 (FITC)
| Brand: | Abnova |
| Reference: | MAB4582 |
| Product name: | IL2RA monoclonal antibody, clone MEM-181 (FITC) |
| Product description: | Mouse monoclonal antibody raised against native IL2RA. |
| Clone: | MEM-181 |
| Isotype: | IgG1 |
| Gene id: | 3559 |
| Gene name: | IL2RA |
| Gene alias: | CD25|IDDM10|IL2R|TCGFR |
| Gene description: | interleukin 2 receptor, alpha |
| Immunogen: | Native purified IL2RA from PHA-activated peripheral blood leucocytes. |
| Form: | Liquid |
| Recommend dilutions: | Flow Cytometry (20 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.2% BSA, 0.09% sodium azide) |
| Storage instruction: | Store in the dark at 4°C. Do not freeze. Avoid prolonged exposure to light. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Conjugate tag: | FITC |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Specificity: | This antibody reacts with CD25 (Interleukin-2 receptor alpha chain), a 55 KDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Surface staining of human PBMC with IL2RA monoclonal antibody, clone MEM-181 (FITC) (Cat # MAB4582). The mononuclear cells were isolated from human peripheral blood, devided in aliquots for duplicate analysis and stimulated with PHA for 2 days. Panel A, C : staining with the IL2RA monoclonal antibody, clone MEM-181 (Cat # MAB0882). Panel B, D : staining with the standard IL2RA monoclonal antibody. |
| Applications: | Flow Cyt |
| Shipping condition: | Blue Ice |
| Publications: | Advances in interleukin 2 receptor targeted treatment.Morris JC, Waldmann TA. Ann Rheum Dis. 2000 Nov;59 Suppl 1:i109-14. |