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Data sheet of IgE monoclonal antibody, clone BE5 (PE)
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Mouse |
| Applications | ELISA,Flow Cyt |
More info about IgE monoclonal antibody, clone BE5 (PE)
| Brand: | Abnova |
| Reference: | MAB4492 |
| Product name: | IgE monoclonal antibody, clone BE5 (PE) |
| Product description: | Mouse monoclonal antibody raised against IgE. |
| Clone: | BE5 |
| Isotype: | IgG |
| Immunogen: | Human IgE. |
| Form: | Liquid |
| Concentration: | 0.1 mg/mL |
| Recommend dilutions: | Flow Cytometry (5 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.2% BSA, 0.09% sodium azide) |
| Storage instruction: | Store in the dark at 4°C. Do not freeze. Avoid prolonged exposure to light. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Conjugate tag: | PE |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Antigen species / target species: | Human |
| Specificity: | This antibody reacts with human IgE; it recognizes an epitope different from the ones recognized by MAB6451 and MAB6452 antibodies to IgE. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Flow Cytometry analysis of basophil activation upon stimulation of normal (heparin-treated) whole blood with combination of IL-3 and Goat anti-IgE polyclonal antibody. Combination of IgE monoclonal antibody, clone BE5 (FITC) (Cat # MAB4491) and IgE monoclonal antibody, clone BE5 (PE) (Cat # MAB4492)-Dyomics 647 was used (analysis in basophil wIDO1w). Fig. A - staining of non-stimulated (control) sample. Fig. B - staining of IgE stimulated sample. |
| Applications: | ELISA,Flow Cyt |
| Shipping condition: | Blue Ice |
| Publications: | IgE isotype determination: epsilon-germline gene transcription, DNA recombination and B-cell differentiation.Gould HJ, Beavil RL, Vercelli D. Br Med Bull. 2000;56(4):908-24. |