Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Human |
Host species | Mouse |
Applications | ELISA,Flow Cyt |
Brand: | Abnova |
Reference: | MAB4492 |
Product name: | IgE monoclonal antibody, clone BE5 (PE) |
Product description: | Mouse monoclonal antibody raised against IgE. |
Clone: | BE5 |
Isotype: | IgG |
Immunogen: | Human IgE. |
Form: | Liquid |
Concentration: | 0.1 mg/mL |
Recommend dilutions: | Flow Cytometry (5 ug/mL) The optimal working dilution should be determined by the end user. |
Storage buffer: | In PBS (0.2% BSA, 0.09% sodium azide) |
Storage instruction: | Store in the dark at 4°C. Do not freeze. Avoid prolonged exposure to light. Aliquot to avoid repeated freezing and thawing. |
Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Conjugate tag: | PE |
Product type: | Primary antibodies |
Host species: | Mouse |
Antigen species / target species: | Human |
Specificity: | This antibody reacts with human IgE; it recognizes an epitope different from the ones recognized by MAB6451 and MAB6452 antibodies to IgE. |
Reactivity: | Human |
Application image: | ![]() |
Application image note: | Flow Cytometry analysis of basophil activation upon stimulation of normal (heparin-treated) whole blood with combination of IL-3 and Goat anti-IgE polyclonal antibody. Combination of IgE monoclonal antibody, clone BE5 (FITC) (Cat # MAB4491) and IgE monoclonal antibody, clone BE5 (PE) (Cat # MAB4492)-Dyomics 647 was used (analysis in basophil wIDO1w). Fig. A - staining of non-stimulated (control) sample. Fig. B - staining of IgE stimulated sample. |
Applications: | ELISA,Flow Cyt |
Shipping condition: | Blue Ice |
Publications: | IgE isotype determination: epsilon-germline gene transcription, DNA recombination and B-cell differentiation.Gould HJ, Beavil RL, Vercelli D. Br Med Bull. 2000;56(4):908-24. |