Brand: | Abnova |
Reference: | MAB3621 |
Product name: | Kinesin (heavy chain) monoclonal antibody, clone KN-02 |
Product description: | Mouse monoclonal antibody raised against native Kinesin (heavy chain). |
Clone: | KN-02 |
Isotype: | IgM |
Immunogen: | Native purified porcine Kinesin (heavy chain). |
Form: | Liquid |
Concentration: | 1 mg/mL |
Recommend dilutions: | The optimal working dilution should be determined by the end user. |
Storage buffer: | In PBS, pH 7.4 (0.09% sodium azide) |
Storage instruction: | Store at 4°C. Do not freeze. |
Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Product type: | Primary antibodies |
Host species: | Mouse |
Antigen species / target species: | Pig |
Specificity: | This antibody recognizes heavy chain of conventional kinesin associated withvesicles and with lower affinity with denaturated molecule. Epitope is located in coiled-coilstalk domain. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies MAB3620, MAB3621 and MAB3622 react with differentsets of fragments. This antibody does not react with kinesin bound to taxol-stabilized microtubules. |
Reactivity: | Human,Mouse,Pig,Rat |
Application image: |  |
Application image note: | Immunofluorescence staining of vesicles (red) in RBL-2H3 (rat basophilic leukemia cell line) using Kinesin (heavy chain) monoclonal antibody, clone KN-02 (Cat # MAB3621). Nuclei were stained with DAPI (blue). |
Applications: | ICC,IF |
Shipping condition: | Blue Ice |
Publications: | Monoclonal antibodies KN-02 and KN-03 against the heavy chain of kinesin.Macurek L, Draberova E, Richterova V, Bohm KJ, Draber P. Hybrid Hybridomics. 2002 Dec;21(6):457-62. |