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Data sheet of PRKACA monoclonal antibody, clone 6D2.1
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Mouse |
| Applications | WB,Dot |
More info about PRKACA monoclonal antibody, clone 6D2.1
| Brand: | Abnova |
| Reference: | MAB3591 |
| Product name: | PRKACA monoclonal antibody, clone 6D2.1 |
| Product description: | Mouse monoclonal antibody raised against synthetic peptide of PRKACA. |
| Clone: | 6D2.1 |
| Isotype: | IgG |
| Gene id: | 5566 |
| Gene name: | PRKACA |
| Gene alias: | MGC102831|MGC48865|PKACA |
| Gene description: | protein kinase, cAMP-dependent, catalytic, alpha |
| Immunogen: | A synthetic peptide corresponding to human PRKACA. |
| Immunogen sequence/protein sequence: | ESPAQNTAHLDQFERIK |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (0.5-1 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS, pH 7.4 (0.09% sodium azide) |
| Storage instruction: | Store at 4°C. Do not freeze. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Mouse |
| Antigen species / target species: | Human |
| Specificity: | This antibody strongly reacts with human proteinkinase A catalytic (PRKACA) alpha subunit, and weakly with PKAc gamma subunit (both around 40 KDa). The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Dot Blot analysis of GST and GST-fusion proteins using PRKACA monoclonal antibody, clone 6D2.1 (Cat # MAB3591) and GST monoclonal antibody, clone S-tag-05 (Cat # MAB3651). The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot. Lane 1 : GST-Akt1. Lane 2 : GST-Akt2. Lane 3 : GST-Akt3. Lane 4 : GST-PKAc alpha. Lane 5 : GST-PKAc beta. Lane 6 : GST-PKAc gamma. Lane 7 : GST-MEK 1. Lane 8 : GST. |
| Applications: | WB,Dot |
| Shipping condition: | Blue Ice |
| Publications: | Monoclonal antibody BF-06 against the heavy chain of clathrin.Macurek L, Zikova M, Draberova E, Draber P. Folia Biol (Praha). 2003;49(6):238-40. |